Zocchi Laboratory for Molecular Biophysics
Misc

DNA Calculator

5' to 3' Sequence:

Mismatch Number:   (Required for "Primer Calculation based on Stratagene Method" only)

         


 

Protein Concentration Calculator (Bradford Assay)

Protocol:
1) 10ul protein sample + 200ul diluted protein assay buffer.
2) Use 200ul for measurement.
3) Incubate >= 5 minutes.
4) Make sure that no bubbles exist.

Reading of Blank:    

Reading of Protein:   

MW of Protein:       (kDa)

Concentration =      (ug/ml)   or    (uM)

 

Useful External Links:

Oligo Calculator Calculate the melting temperature of the primer that you're trying to design
Unit Conversion Converts units such as length, temperature, energy, and even curreny
Quikchange Primer Tm Stratagene Primer Calculator
Restriction Sites Locator Locate Restriction Sites of your DNA sequences with Webcutter 2.0
DNA Sequencing UCLA Sequencing & Genotyping Core (Gonda Center Rm. 5309)
Sequence Alignment EMBOSS Pairwise Alignment Algorithms (more tools at EMBL-EBI)
Protein Data Bank Database of 3D macromolecule structures solved by X-ray or NMR

 

 

 

Copyright @ Zocchi Lab @ Physics Department @ UCLA [Updated: 02/08/2010]